Journal: Molecular pharmaceutics

Article Title: In Situ Vaccination with Cowpea vs Tobacco Mosaic Virus against Melanoma

doi: 10.1021/acs.molpharmaceut.8b00316

Figure Lengend Snippet: Antibodies Used for Flow Cytometry

Article Snippet: Flow cytometry analyses were performed on a BD LSR II flow cytometer (BD Biosciences), and data was analyzed using the FlowJo software (Tree Star Inc.). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 dye marker vendor innate panel catalog no. clone/host isotype concentration pacific blue CD4S BioLegend 103126 30-F11 Rat IgG2b, K 0.5 mg/mL FITC CD11b BioLegend 10120S Ml/70 Rat IgG2b, K 0.5 mg/mL PE CD80 BioLegend 104707 16–10A1A Hamster IgG 0.2 mg/mL PE-Cy7 CD86 BioLegend 10S014 GL-1 Rat IgG2a, K 0.2 mg/mL APC I-A/I-E (=MHCII) BioLegend 107613 MS/114.1S.2 Rat IgG2b, K 0.2 mg/mL APC/Cy7 Ly-6G BioLegend 127623 1A8 Rat IgG2a, K 0.2 mg/mL APC CD11c BioLegend 10120S N418 A Hamster 0.2 mg/mL PE F4/80 BioLegend 123109 BM8 Rat IgG2a, K 0.2 mg/mL PE/Cy7 Ly6C BioLegend 128018 HK1.4 Rat IgG2c, K 0.2 mg/mL BV60S NK1.1 BioLegend 108737 PK136 IgG2a, k 0.2 mg/mL dye marker vendor adaptive panel catalog no. clone/host isotype concentration pacific blue CD45 BioLegend 103126 30-F11 Rat IgG2b, K 0.5 mg/mL FITC CD4 BioLegend 100405 GK1.5 Rat IgG2b, K 0.5 mg/mL PE CD44 BioLegend 103007 1M7 Rat IgG2b, K 0.2 mg/mL PE-Cy7 CD62L (L-selectin) BioLegend 104417 MEL-14 Rat IgG2a, K 0.2 mg/mL APC CD8 α BioLegend 100712 S3–6.7 Rat IgG2a, K 0.2 mg/mL APC/Cy7 CD3 ε BioLegend 100330 14S-2C11 A. Hamster IgG 0.2 mg/mL Open in a separate window Antibodies Used for Flow Cytometry

Techniques: Marker, Concentration Assay

Journal: Molecular pharmaceutics

Article Title: In Situ Vaccination with Cowpea vs Tobacco Mosaic Virus against Melanoma

doi: 10.1021/acs.molpharmaceut.8b00316

Figure Lengend Snippet: Antibodies Used for Immunohistochemistry

Article Snippet: Flow cytometry analyses were performed on a BD LSR II flow cytometer (BD Biosciences), and data was analyzed using the FlowJo software (Tree Star Inc.). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 dye marker vendor innate panel catalog no. clone/host isotype concentration pacific blue CD4S BioLegend 103126 30-F11 Rat IgG2b, K 0.5 mg/mL FITC CD11b BioLegend 10120S Ml/70 Rat IgG2b, K 0.5 mg/mL PE CD80 BioLegend 104707 16–10A1A Hamster IgG 0.2 mg/mL PE-Cy7 CD86 BioLegend 10S014 GL-1 Rat IgG2a, K 0.2 mg/mL APC I-A/I-E (=MHCII) BioLegend 107613 MS/114.1S.2 Rat IgG2b, K 0.2 mg/mL APC/Cy7 Ly-6G BioLegend 127623 1A8 Rat IgG2a, K 0.2 mg/mL APC CD11c BioLegend 10120S N418 A Hamster 0.2 mg/mL PE F4/80 BioLegend 123109 BM8 Rat IgG2a, K 0.2 mg/mL PE/Cy7 Ly6C BioLegend 128018 HK1.4 Rat IgG2c, K 0.2 mg/mL BV60S NK1.1 BioLegend 108737 PK136 IgG2a, k 0.2 mg/mL dye marker vendor adaptive panel catalog no. clone/host isotype concentration pacific blue CD45 BioLegend 103126 30-F11 Rat IgG2b, K 0.5 mg/mL FITC CD4 BioLegend 100405 GK1.5 Rat IgG2b, K 0.5 mg/mL PE CD44 BioLegend 103007 1M7 Rat IgG2b, K 0.2 mg/mL PE-Cy7 CD62L (L-selectin) BioLegend 104417 MEL-14 Rat IgG2a, K 0.2 mg/mL APC CD8 α BioLegend 100712 S3–6.7 Rat IgG2a, K 0.2 mg/mL APC/Cy7 CD3 ε BioLegend 100330 14S-2C11 A. Hamster IgG 0.2 mg/mL Open in a separate window Antibodies Used for Flow Cytometry

Techniques:

Journal: ACS Biomaterials Science & Engineering

Article Title: Effect of Antigen Structure in Subunit Vaccine Nanoparticles on Humoral Immune Responses

doi: 10.1021/acsbiomaterials.2c01516

Figure Lengend Snippet: (A) Schematic overview of vaccination study. Total IgG (B), IgG1 (C), and IgG2a (D) from vaccinated mice after prime (circles) and after boost (triangles). (E) Antibody affinity of OVA-specific total IgG at 8 weeks. N = 5–6. *, p ≤ 0.05, **, p ≤ 0.01, ***, p ≤ 0.001, ****, and p ≤ 0.0001. ns represent statistically nonsignificant differences.

Article Snippet: For in vivo experiments, horseradish peroxidase (HRP)-conjugated rabbit anti-OVA IgG and goat anti-mouse IgG, IgG1, and IgG2a were purchased from Southern Biotech, and Armenian hamster anti-mouse CD3ε (PerCP), rat anti-mouse CD8a (FITC), rat anti-mouse CD4 (APC/Cy7), rat anti-mouse IFN-γ (PE), rat anti-mouse IL-4 (PE/Cy7), Armenian hamster anti-mouse CD11c (APC/Cy7), Zombie Violet Viability Kit, brefeldin A (1000x), and monensin (1000x) were purchased from Biolegend.

Techniques:

Journal: ACS Biomaterials Science & Engineering

Article Title: Effect of Antigen Structure in Subunit Vaccine Nanoparticles on Humoral Immune Responses

doi: 10.1021/acsbiomaterials.2c01516

Figure Lengend Snippet: (A) Schematic overview of vaccination study. Total IgG (B), IgG1 (C), and IgG2a (D) from vaccinated mice after prime (circles) and after boost (triangles). (E) Antibody affinity of OVA-specific total IgG at 8 weeks. N = 5–6. *, p ≤ 0.05, **, p ≤ 0.01, ***, p ≤ 0.001, ****, and p ≤ 0.0001. ns represent statistically nonsignificant differences.

Article Snippet: For in vivo experiments, horseradish peroxidase (HRP)-conjugated rabbit anti-OVA IgG and goat anti-mouse IgG, IgG1, and IgG2a were purchased from Southern Biotech, and Armenian hamster anti-mouse CD3ε (PerCP), rat anti-mouse CD8a (FITC), rat anti-mouse CD4 (APC/Cy7), rat anti-mouse IFN-γ (PE), rat anti-mouse IL-4 (PE/Cy7), Armenian hamster anti-mouse CD11c (APC/Cy7), Zombie Violet Viability Kit, brefeldin A (1000x), and monensin (1000x) were purchased from Biolegend.

Techniques: